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真龙柚是从沙田柚芽变中选育出的新品种。为了进一步确定芽变系性状的可遗传性,对沙田柚和真龙柚及其无性系子代6个样品进行了RAPD和SSR分析。结果表明,8个RAPD引物共扩增出43条稳定清晰的条带,其中5条具有多态性,多态性比率为11.6%;26对SSR引物共扩增出34条带,只有5条带具有多态性,多态性比率为14.7%;真龙柚样品与沙田柚样品的遗传距离为0.063,它们之间在DNA水平上存在遗传差异。
The real dragon pomelo is a new variety bred from the pomelo bud Shatian. In order to further determine the heritability of the mutant lines, RAPD and SSR analysis were performed on six samples of Shatianyou pomelo and its members. The results showed that a total of 43 stable and clear bands were amplified by 8 RAPD primers, of which 5 were polymorphic with a polymorphism ratio of 11.6%. A total of 26 SSR primers amplified 34 bands and only 5 bands The polymorphic bands were 14.7%. The genetic distance between the samples of true pomelo was 0.063 and the samples of pomelo were 0.063. There was a genetic difference in the DNA level between them.