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目的 :研究肿瘤坏死因子相关凋亡诱导配体 (TRAIL)在病毒诱导的人胰岛 β细胞损伤中的作用。 方法 :用Annexin -V法检测比较柯萨奇B病毒 (CVB)和风疹病毒 (RV)引起的人胰岛 β细胞系CM细胞凋亡 ,再用可溶性TRAIL受体和抗TRAIL单克隆抗体进行阻断实验。结果 :5噬斑形成单位 (PFU)CVB3和CVB4作用CM细胞 5h导致 30 %以上CM细胞凋亡 ;0 0 1PFUCVB3和CVB4作用CM细胞 2 4h导致超过 80 %CM细胞凋亡 ;10PFURV作用CM细胞 2 4h导致 2 5 %CM细胞凋亡 ;4种可溶性TRAIL受体以及抗TRAIL单克隆抗体对CVB所致细胞凋亡有阻断作用。结论 :CM细胞对CVB更为敏感 ,TRAIL参与CVB诱导的CM细胞凋亡。
AIM: To investigate the role of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in virus-induced injury of human pancreatic β-cells. Methods: The apoptosis of human pancreatic β cell line CM cells induced by Coxsackie B virus (CVB) and rubella virus (RV) was detected by Annexin-V assay and then blocked by soluble TRAIL receptor and anti-TRAIL monoclonal antibody experiment. Results: 5-day plaque-forming unit (PFU) induced by CVB3 and CVB4 in CM cells for more than 30% of CM cells, and induced apoptosis in more than 80% of CM cells by 0 0 1 PFUVB3 and CVB4. 10 PFURV-induced CM cells 2 4h lead to 25% CM cell apoptosis; 4 soluble TRAIL receptor and anti-TRAIL monoclonal antibody blocking CVB-induced apoptosis. CONCLUSIONS: CM cells are more sensitive to CVB and TRAIL is involved in the CVB-induced apoptosis of CM cells.