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目的通过犬心肌梗死及缺血再灌注模型，观察碘标β甲基碘苯脂十五烷酸（ＢＭＩＰＰ）检测缺血区存活与坏死心肌的作用及其与局部血流的关系。方法１２条实验犬结扎左回旋支冠状动脉后分成２组，即单纯缺血组与缺血再灌注组。静脉注射１２５ＩＢＭＩＰＰ和９９Ｔｃｍ右旋糖酐微球（ＴＭ），３０ｍｉｎ后处死实验犬，摘除心脏，分别进行ＥＶＡＮＳ蓝和ＮＢＴ染色，确定正常、缺血和坏死心肌范围，并分别测定同一组织块１２５Ｉ和９９Ｔｃｍ的放射性计数，各局部心肌血流量与ＢＭＩＰＰ摄取量以左心室组织块峰计数的百分率表示。结果在单纯缺血组中，缺血心肌局部ＢＭＩＰＰ的摄取量为（６７±２３）％，明显高于局部血流（４２±１９）％，但与正常心肌组比较，局部血流和ＢＭＩＰＰ摄取量均明显减低；坏死心肌局部血流为（２６±１１）％，与ＢＭＩＰＰ摄取量（２５±１３）％差异无显著性，但明显低于缺血心肌组的局部血流和ＢＭＩＰＰ浓聚量；缺血心肌再灌注后，无论缺血或坏死心肌，局部血流均见明显恢复，但局部ＢＭＩＰＰ的摄取量与单纯缺血组比较无明显增加。结论在血流灌注减低区，ＢＭＩＰＰ浓聚量相对增高可能是缺血区心肌存活的标志；而血流量与ＢＭＩＰＰ浓聚量一致性严重减低的部位?
Objective To observe the effect of myocardial iodine β-methyliodobenzenepentadecanoic acid (BMIPP) on ischemic and necrotic myocardium and its relationship with local blood flow in canine myocardial infarction and ischemia-reperfusion model. Methods Twelve experimental dogs were ligated with left circumflex coronary artery and divided into two groups, ie, ischemia-reperfusion group and ischemia-reperfusion group. 125IBMIPP and 99Tcmdehydroglucose microspheres (TM) were injected intravenously. After 30min, the dogs were sacrificed and hearts were removed. EVANS blue and NBT staining were performed respectively to determine the myocardial areas of normal, ischemic and necrotic areas. Radiological counts of 99 Tcm, each local myocardial blood flow and BMIPP uptake are expressed as a percentage of the peak of the left ventricular mass. Results In the ischemia group, the uptake of BMIPP in the ischemic myocardium was (67 ± 23)%, which was significantly higher than that of the local blood flow (42 ± 19%). However, compared with the normal myocardial group, the local blood flow and BMIPP uptake (26 ± 11)% of the necrotic myocardium, but no significant difference with BMIPP uptake (25 ± 13)%, but significantly lower than the local blood flow and BMIPP concentration in ischemic myocardium After reperfusion of ischemic myocardium, no significant difference was observed in the local blood flow between ischemic and necrotic myocardium. However, the uptake of local BMIPP was not significantly increased compared with the ischemia group. Conclusion In the area of hypoperfusion, the relative increase of BMIPP concentration may be a marker of myocardial survival in ischemic area. However, the area where the consistency of blood flow and BMIPP concentration is severely reduced?