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A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography (HPAEC)has been developed with integrated pulsed amperometric detection (IPAD).Diouex AS18 (250mm × 2mm) and AG18 (50 mm× 2 mm) columns and 11 mmol/L NaOH solution were used for separation.Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio (S/N).Utilizing an optimized waveform,the repeatability (intra-day) precision and intermediate (inter-day) precision are obtained with relative standard deviation (RSD) of 1.88,2.27,respectively.The limit of quantification (LOQ) and limit of detection (LOD) were found to be 9.70,3.0 ng/mL,respectively,with correlation coefficients of 0.9992 over concentration range 0.01-10 μg/mL.The present method was successfully applied to the determination of zidovudine in human plasma.The recoveries of plasma sample spiked by 0.7 μg/mL,2.7 μg/mL obtained were 95.3-101.5％,with relative standard deviation (RSD) of 2.54％,2.21％,respectively.