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为了研究高氟低碘对大鼠甲状腺细胞DNA损伤的影响,试验给离乳Wistar大鼠饲喂不同含量氟、碘的饮水和饲料,在大鼠20月龄时采取甲状腺组织,采用单细胞凝胶电泳技术检查其DNA的损伤情况。结果表明:与对照组相比,高氟组、低碘组和高氟低碘组的老年大鼠甲状腺细胞拖尾率显著增加,拖尾长度显著增长;高氟组大鼠甲状腺细胞DNA损伤主要是Ⅱ级、Ⅲ级(Ⅱ级为39.13%、Ⅲ级为47.83%),而低碘组的甲状腺细胞DNA损伤的三个级别所占比率几乎平均,且Ⅱ级损伤较高;高氟低碘组的细胞损伤以Ⅲ级损伤为主。说明高氟、低碘、高氟低碘都影响了大鼠甲状腺的DNA损伤。
In order to study the effect of high fluoride and low iodine on the DNA damage of thyrocytes in rats, the Wistar rats were fed with drinking water and feed containing different levels of fluorine and iodine. Thyroid tissues were collected at 20 months of age in rats. Gel electrophoresis technology to check the DNA damage. The results showed that compared with the control group, the thyroid cells in the high fluoride group, the low iodine group and the high fluoride and low iodine group had significantly increased tailing rate and increased the trailing length of the thyroid cells. The DNA damage in the thyroid cells in the high fluoride group was mainly Grade Ⅱ, grade Ⅲ (grade of 39.13%, grade of grade 47.83%), while the iodine group, thyroid cell DNA damage in the three levels accounted for almost the average, and grade Ⅱ higher damage; high fluoride Group of cell damage to grade Ⅲ-based injury. High fluoride, low iodine, high fluoride and low iodine affect DNA damage of thyroid in rats.