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从洞庭湖防洪大堤护坡和湖滩捕捉试鼠,在实验室做无选择性摄食试验。将试鼠雌雄分开,随机分组,配制0.000 2%溴敌隆毒饵进行不同时间的攻毒试验。用SPSS进行几率单位加权回归法(Bliss法)的计算,探讨不同食毒期与对应死亡率之间的关系。结果表明,东方田鼠性别之间对溴敌隆的敏感性差异无统计学意义,故将雌雄数据合并计算,得毒力回归方程y=0.07+2.65x,LFP50、LFP98、LFP99及其95%置信限分别是0.94(0.43~1.33)、5.63(3.51~20.83)d和7.14(4.15~33.52)d。按WHO修订以致死99%敏感靶标鼠种的食毒期(LFP99)取整天数作为抗药性检验标准,洞庭湖区东方田鼠对0.000 2%的溴敌隆毒饵食毒期超过8 d存活为产生抗药性。
From the Dongting Lake flood embankment slope and the beach capture test rats, in the laboratory to do non-selective feeding test. The test rats were separated from each other by male and female randomly, and 0.000 2% bromadiolone bait was prepared for the challenge test at different times. SPSS was used to calculate the odds unit weighted regression method (Bliss method) to explore the relationship between different food poisoning and the corresponding mortality. The results showed that there was no significant difference in the sensitivity to the content of bromadiolone between male and female voles. Therefore, the male and female data were combined to calculate the virulence regression equation y = 0.07 + 2.65x, LFP50, LFP98, LFP99 and 95% confidence The limits were 0.94 (0.43-1.33), 5.63 (3.51-20.83) d and 7.14 (4.15-33.52) d, respectively. According to the WHO revised as 99% lethal target sensitive mice (LFP99) take the number of days rounded off as the drug resistance test standard, Dongting Lake area of Microtus fortis 0.0002% bromadiolone poisoning period of more than 8 d survival to produce Resistance.