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目的探讨中波紫外线照射对实验性矽肺大鼠氧化损伤的影响。方法健康成年SD雄性大鼠32只,随机分为4组,通过一次性非暴露式气管注入二氧化硅粉尘混悬液及施加中波紫外线照射后,采用比色法测定大鼠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(HYP)和血清中铜兰蛋白(CP);测量大鼠体重增长量、脏器湿重。结果(1)注入二氧化硅粉尘后大鼠肺组织匀浆中SOD活性明显降低(P<0.01),肺匀浆中MDA含量显著升高(P<0.01);肺组织匀浆中HYP含量和血清中CP活性显著升高(P<0.05)。(2)照射中波紫外线后大鼠肺组织匀浆中SOD活性降低(P<0.01);MDA、HYP和CP在照射中波紫外线后均无变化(P>0.05)。(3)中波紫外线和二氧化硅粉尘对大鼠肺组织匀浆中SOD、HYP和血清中CP存在交互作用(P<0.01);中波紫外线和二氧化硅粉尘对大鼠肺组织匀浆中MDA存在交互作用(P<0.05)。结论二氧化硅粉尘可致大鼠肺组织氧化损伤,导致肺组织纤维化;而中波紫外线的照射可以降低矽肺模型大鼠肺组织的氧化损伤程度,减轻模型鼠肺组织纤维化程度。
Objective To investigate the effects of UVB irradiation on oxidative damage in experimental silicosis rats. Methods Thirty-two SD adult male SD rats were randomly divided into four groups. After a single non-exposure tracheal injection of silica dust suspension and UVB irradiation, the contents of superoxide (SOD), malondialdehyde (MDA), hydroxyproline (HYP) and serum ceruloplasmin (CP). The body weight gain and organ weight were measured. Results (1) SOD activity in lung homogenate was significantly decreased (P <0.01), and MDA content in lung homogenate was significantly increased (P <0.01). The content of HYP in lung homogenate Serum CP activity was significantly increased (P <0.05). (2) The activity of SOD in lung homogenate of rats was decreased after UVB exposure (P <0.01); MDA, HYP and CP were not changed after UVB irradiation (P> 0.05). (3) UVB and silica dust had interaction on SOD, HYP and CP in serum (P <0.01); UVB and silica dust in rat lung homogenate MDA in the interaction (P <0.05). Conclusion Silica dust can cause oxidative damage of lung tissue in rats, leading to lung fibrosis. UVB irradiation can reduce the degree of oxidative damage of lung tissue in silicotic model rats and reduce the degree of lung fibrosis in model mice.