论文部分内容阅读
本文报道以小鼠βNGF为抗原免疫家兔,制备βNGF抗血清,用于Western印迹法,测定结果βNGF在分子量约14kDa处显一条带。亲和层析纯化抗血清得到βNGF抗体。采用鸡胚背根神经节培养法测定了βNGF及其抗体的生物学活性
This paper reports the mouse βNGF as antigen immunized rabbits, βNGF antiserum was prepared for Western blot, the results of βNGF molecular weight of about 14kDa showed a band. Affinity chromatography purification of antisera to obtain βNGF antibody. The biological activity of βNGF and its antibody was determined by chicken embryo dorsal root ganglion culture