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本文报道以小鼠βＮＧＦ为抗原免疫家兔，制备βＮＧＦ抗血清，用于Ｗｅｓｔｅｒｎ印迹法，测定结果βＮＧＦ在分子量约１４ｋＤａ处显一条带。亲和层析纯化抗血清得到βＮＧＦ抗体。采用鸡胚背根神经节培养法测定了βＮＧＦ及其抗体的生物学活性
This paper reports the mouse βNGF as antigen immunized rabbits, βNGF antiserum was prepared for Western blot, the results of βNGF molecular weight of about 14kDa showed a band. Affinity chromatography purification of antisera to obtain βNGF antibody. The biological activity of βNGF and its antibody was determined by chicken embryo dorsal root ganglion culture