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The nucleocapsid(N)protein of severe acute respiratory syndrome-coronavirus(SARS-CoV)is a majorvirion structural protein.In this study,two epitopes(N1 and N2)of the N protein of SARS-CoV werepredicted by bioinformatics analysis.After immnnization with two peptides,the peptides-specific antibodieswere isolated from the immunized rabbits.The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E.coli expressed Nprotein of SARS-CoV.Furthermore,itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome(SARS)patients.The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization,which will be useful for the study of SARS-CoV.
The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a major virion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV were predicted by bioinformatics analysis. After immnnization with two peptides, the peptides-specific antibodies were isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-induced polyclonal antibodies had a high affinity to bind to E. coli expressed Nprotein of SARS-CoV. Stillrther, it was confirmed that N1 peptide- specific IgG antibodies were detectable in the sera of severe acute respiratory syndrome (SARS) patients. These results indicate that an epitope of the N protein has been identified and N protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS- CoV.