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目的:全反式维甲酸(all-trans retinoic acid,ATRA)治疗急性早幼粒细胞白血病,引起白血病细胞与内皮细胞之间黏附增加,是发生维甲酸综合征的重要原因。本文观察雷公藤红素对这种黏附增加的影响。方法:用流式细胞术检测雷公藤红素对急性早幼粒细胞白血病细胞系NB4和人脐静脉内皮细胞(humanumbilical vascular endothelial cell,HUVEC)在ATRA刺激下表达黏附分子的影响。用细胞黏附功能实验,检测雷公藤红素对ATRA导致的上述两种细胞之间黏附增加的影响。结果:ATRA能引起NB4细胞表面细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)表达增加,但可被雷公藤红素明显抑制(P<0.01)。NB4细胞上清液能刺激HUVEC表达ICAM-1(P<0.05);而被ATRA处理过的NB4细胞上清液能明显刺激HUVEC表达选择素E(E-selectin)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)和ICAM-1等黏附分子(P<0.01),雷公藤红素对其抑制率分别达25.3%、42.4%和61.0%。ATRA导致上述两种细胞之间黏附增加,雷公藤红素对其完全抑制。结论:雷公藤红素能抑制ATRA导致的白血病细胞与内皮细胞黏附,有可能用于维甲酸综合征的治疗。
OBJECTIVE: All-trans retinoic acid (ATRA) treatment of acute promyelocytic leukemia, causing increased adhesion between leukemia cells and endothelial cells, is an important cause of retinoic acid syndrome. This article to observe the impact of tripterine on the increase in adhesion. Methods: Flow cytometry was used to detect the effect of tripterine on the expression of adhesion molecules in acute promyelocytic leukemic cell line NB4 and human umbilical vascular endothelial cells (HUVECs) stimulated by ATRA. Cell adhesion assay was used to determine the effect of tripterine on ATRA-induced increased adhesion between the two cells. Results: ATRA could increase the expression of intercellular adhesion molecule-1 (ICAM-1) on NB4 cells, but could be significantly inhibited by tripterine (P <0.01). The supernatant of NB4 cells could stimulate the expression of ICAM-1 in HUVECs (P <0.05), while the supernatant of NB4 cells treated with ATRA could obviously stimulate the expression of E-selectin and vascular cell adhesion molecule-1 vascular cell adhesion molecule-1, VCAM-1) and ICAM-1 (P <0.01). The inhibitory rates of tripterine were 25.3%, 42.4% and 61.0%, respectively. ATRA caused an increase in adhesion between the two cells, which was completely inhibited by triproline. Conclusion: Tripterine can inhibit the adhesion of leukemia cells and endothelial cells induced by ATRA, and may be used for the treatment of retinoic acid syndrome.