前言 50年代初,国外已用聚乙二醇(Polyethy-lene glycol以下称PEG,)包埋制片进行组织化学染色。近年来报导用PEG沉淀浓缩病毒和蛋白质等对其活性并无影响。因此我们也建立PEG包埋制片技术并经改进后试用于正常小鼠组织切片染色及乙脑和Sindbis病毒感染鼠脑组织的免疫酶组化染色。一、PEG包埋剂配方配方:PEG1000和4000以3:1比例混合(夏季用等量混合),加热熔化,置45°—56℃恒温箱备用。 Preface In the early 1950s, foreign countries have polyethylene glycol (Polyethy-lene glycol, hereinafter referred to as PEG,) embedded film for histochemical staining. In recent years, it has been reported that the concentration of viruses and proteins with PEG precipitation has no effect on its activity. Therefore, we also established a PEG-based embedded preparation technique and improved it for tryptic staining of normal mouse tissue sections and brain tissues infected with JE and Sindbis virus. First, PEG embedding formula formula: PEG1000 and 4000 mixed at a ratio of 3: 1 (summer mixed with the same amount), heated to melt, set 45 ° -56 ℃ incubator standby.