用两种针对旋毛虫幼虫可溶性抗原的单克隆抗体(MAb 3G6和MAb 3 E10),通过ELISA和IFA检测旋毛虫和伪旋毛虫间的抗原差异。 ELISA结果证明,MAb 3G6与旋毛虫、南方旋毛虫、北方旋毛虫和伪旋毛虫幼虫的盐水可溶性抗原均可发生阳性反应,其消光值(波长492nm,空白对照0.03)分别为0.988,0.86,0.79,0.81;相同条件下,用MAb3E10检测上述四种旋毛虫,其消光值分别为0.84,0.9,0.695,0.024,表明MAb 3E10同 Antigen differences between Trichinella spiralis and Pseudostrichia were detected by ELISA and IFA using two monoclonal antibodies to the Trichinella spiralis larvae soluble antigen (MAb 3G6 and MAb 3 E10). ELISA results showed that MAb 3G6 and Trichinella spiralis, Trichinella spiralis, Trichinella spiralis and Trichinella spiralis larvae saltwater soluble antigen positive reaction can occur, the extinction value (wavelength 492nm, blank control 0.03) were 0.988,0.86,0.79 , 0.81; Under the same conditions, the above four kinds of Trichinella spiralis were detected by MAb3E10, and their extinction values ​​were 0.84, 0.9, 0.695 and 0.024, respectively, indicating that MAb 3E10