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目的 探讨碱性成纤维细胞生长因子 (bFGF)、血管内皮细胞生长因子 (VEGF)和血小板衍化内皮细胞生长因子 (PD ECGF)与肝癌微血管密度及门静脉癌栓形成的关系。方法 用免疫组化法对 6 1例手术切除的肝细胞癌 (HCC)标本分别作抗肿瘤血管内皮细胞染色以及抗bFGF、VEGF、PD ECGF染色。结果 HCC组织中bFGF、VEGF和PD ECGF表达阳性率分别为 45 9%、75 4%和 70 5 %。HCC组织中bFGF、VEGF和PD ECGF表达均阴性、其中之一表达阳性、其中两种表达阳性及三种均表达阳性者 ,伴有门静脉癌栓的阳性率分别为 16 7%、2 5 0 %、5 4 2 %和 6 3 2 % ,趋势卡方检验差异有显著意义 (P <0 .0 5 ) ;HCC组织中的MVD计数分别为 99± 5 6、113± 2 7、140± 47和 194± 5 2 ,方差分析差异有非常显著差异 (P <0 .0 0 1)。结论 随着HCC组织中血管生长因子表达阳性数目的增多 ,其肿瘤血管生成能力明显增大 ,形成门静脉癌栓的机率亦增加。
Objective To investigate the relationship between basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD ECGF) and the microvessel density and portal vein tumor thrombosis in hepatocellular carcinoma. Methods Immunohistochemical method was used to detect the staining of anti-tumor vascular endothelial cells and the anti-bFGF, VEGF and PD ECGF in 61 specimens of surgically resected hepatocellular carcinoma (HCC). Results The positive rates of bFGF, VEGF and PD ECGF expression in HCC tissues were 45.9%, 75.4% and 70.5%, respectively. The expression of bFGF, VEGF and PD ECGF was negative in HCC tissues, one of them was positive, two were positive and the other three were positive. The positive rate of portal vein tumor thrombus was 16 7% and 25% respectively. , 542 and 632% were significantly different from the trend chi-squared test (P < 0.05); the MVD counts in HCC tissue were 99 ± 5 6, 113 ± 2 7, 140 ± 47 and 194 ± 5 2 , there was a significant difference in variance analysis (P <0. 0 0 1). Conclusion With the increase in the number of positive expression of angiogenic growth factors in HCC tissue, the tumor angiogenesis ability is significantly increased, and the probability of forming portal vein tumor thrombus is also increased.