论文部分内容阅读
目的:研究疏肝和胃汤水提醇沉液对肝郁脾虚功能性消化不良(FD)大鼠胃肠动力、胃动素(MTL)、胃泌素(GAS)、胃促生长素(Ghrelin)的影响,探讨其治疗FD的疗效及机制。方法:将48只SPF级SD大鼠随机分为两组,分别为空白对照组(A组,12只,雌雄各半)和模型组(M组,36只,雌雄各半),空白对照组不做任何处理,模型组采用复合病因造模法(不规则饮食+饮酸+夹尾)复制肝郁脾虚功能性消化不良大鼠模型。连续造模14 d后,将M组随机分为3组,分别为模型对照组(B组),莫沙必利组(C组),疏肝和胃组(D组),每组12只,雌雄各半。造模完成后,连续给药两周,C组和D组分别给予灌服莫沙必利和疏肝和胃汤水提醇沉液;A组和B组给予等体积蒸馏水。观察动物的一般状态,给药结束后测定胃内残存率和小肠推进率。采用酶联免疫法测定血清MTL、GAS、Ghrelin的水平。结果:与正常对照组比较,模型对照组胃内残存率明显增加,小肠推进率明显降低,MTL、GAS、Ghrelin水平均显著降低;与模型对照组比较,莫沙必利组与疏肝和胃组均能降低模型大鼠胃内残存率和提高小肠推进率,两药物组均能升高MTL、GAS、Ghrelin水平。结论:疏肝和胃汤水提醇沉液对治疗功能性消化不良有一定的疗效,其机制可能是促进胃肠动力和调节MTL、GAS及Ghrelin水平。
Objective: To investigate the effects of Shugan Hewei decoction on gastric motility, motilin (MTL), gastrin (GAS) and ghrelin in rats with functional dyspepsia (FD) Of the impact of its treatment of FD efficacy and mechanism. Methods: Forty eight Sprague Dawley rats were randomly divided into two groups: blank control group (A group, 12 rats, male and female) and model group (M group, 36 rats, male and female respectively), blank control group Without any treatment, model group using compound etiology modeling (irregular diet + drinking acid + clip tail) copy of liver dysfunction syndrome rat model of liver spleen deficiency. After continuous modeling for 14 days, M groups were randomly divided into three groups: model control group (B group), mosapride group (C group), Shugan and stomach group (D group), 12 rats in each group , Male and female half. After modeling, continuous administration for two weeks, C group and D group were given Mosapride and Shugan and stomach soup alcohol precipitation sedimentation; Group A and B were given equal volume of distilled water. The general state of the animals was observed, and the residual gastric rate and intestinal propulsion rate were measured after administration. Serum MTL, GAS and Ghrelin levels were measured by enzyme-linked immunosorbent assay. Results: Compared with the normal control group, the gastric residual rate in the model control group increased significantly, the small intestine propulsion rate decreased significantly and the levels of MTL, GAS and Ghrelin decreased significantly. Compared with the model control group, Both groups can reduce gastric remnant rate and improve the small intestine propulsion rate in model rats, and both groups can increase MTL, GAS and Ghrelin levels. Conclusion: Shugan and stomach soup alcohol extract fluid treatment of functional dyspepsia have a certain effect, and its mechanism may be to promote gastrointestinal motility and regulate MTL, GAS and Ghrelin levels.