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血小板显示吞噬细胞的特性,这有益于机体—吞噬和消灭异物。但血小板与细菌及其代谢产物相互作用可导致严重并发症。我们建立了以细菌诱导血小板聚集的方法,已观察了一些药物的作用。 材料与方法 (一)血小板血浆的制备 体重1.5～2.5kg的雄兔及150～250g雄性大鼠,按文献4方法制备枸橼酸血小板血浆,使含3.4～4.5×10~3个/ml(540nm波长时透光度为24～30％)。 (二)细菌悬液的制备:金黄色葡萄球菌(金葡菌)26108,209p、1～8和15号等菌株。枯草杆菌
Platelets show phagocytic properties that benefit the body - phagocytosis and elimination of foreign bodies. However, the interaction of platelets with bacteria and their metabolites can lead to serious complications. We have established a method of bacterial-induced platelet aggregation and the effects of some drugs have been observed. Materials and methods (a) Preparation of platelet plasma Male rabbits weighing 1.5-2.5 kg and male rats of 150-250 g were prepared according to the method of literature 4, and the concentration of platelet-rich plasma containing 3.4-4.5 × 10-3 / ml ( 540nm wavelength transmittance of 24 ~ 30%). (B) the preparation of bacterial suspension: Staphylococcus aureus (Staphylococcus aureus) 26108,209p, 1 to 8 and 15 strains. Bacillus subtilis