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目的:探讨HPVL1壳蛋白在不同宫颈病变中的表达及其临床意义并提示其预后。方法:应用赛泰试剂盒,用免疫组化方法检测不同宫颈病变中HPVL1壳蛋白的表达。结果:慢性宫颈炎和宫颈鳞状细胞癌中HPVL1壳蛋白检测阴性。HPVL1壳蛋白在CINI、CINII和CINIII级中的阳性表达率分别为79.5%、50%和12.5%,CINII级中HPVL1壳蛋白表达明显低于CINI;CINIII明显低于CINII;差异有统计学意义(P<0.01)。结论:HPVL1壳蛋白的检测可预测宫颈病变的发展趋势,尤其是对CINI和CINII的宫颈病变的治疗具有指导价值。人乳头状瘤病毒(HPV)感染是引发宫颈癌的重要因素,但是大部分HPV感染是亚临床的、自限性的,可造成不同的子宫颈病变,并不是所有的HPV感染都会进展为宫颈癌。目前临床常用的诊断技术尚无法预测HPV感染引起子宫颈病变的转归,因而大量患者被过度治疗。HPVL1壳蛋白是近年来发现的一种反映HPV感染预后的重要生物标记物。作者应用最新引进的“赛泰”HPVL1壳蛋白检测试剂盒,采用免疫组化方法检测不同级别宫颈鳞状上皮内瘤变中HPVL1壳蛋白的表达,旨在探讨预测宫颈鳞状上皮内瘤变进展中的价值。
Objective: To investigate the expression of HPVL1 capsid protein in different cervical lesions and its clinical significance and prognosis. Methods: Using Saitai kit, the expression of HPVL1 capsid protein in different cervical lesions was detected by immunohistochemistry. Results: HPV16 capsid protein was negative in chronic cervicitis and cervical squamous cell carcinoma. The positive expression rates of HPVL1 capsid protein in CINI, CINII and CINIII were 79.5%, 50% and 12.5%, respectively. The expression of HPVL1 capsid protein in CINII was significantly lower than that in CINI; CINIII was significantly lower than that in CINII P <0.01). Conclusion: The detection of HPVL1 capsid protein can predict the development of cervical lesions, especially for the treatment of cervical lesions CINI and CINII. Human papillomavirus (HPV) infection is an important factor in causing cervical cancer, but most HPV infections are subclinical and self-limiting and can cause different cervical lesions. Not all HPV infections progress to cervical cancer. At present, the diagnostic techniques commonly used in clinic are still unable to predict the outcome of cervical lesions caused by HPV infection, so a large number of patients are over-treated. HPVL1 capsid protein is an important biomarker that has been found in recent years to reflect the prognosis of HPV infection. The authors applied the newly introduced “Saitai ” HPVL1 capsid protein detection kit, using immunohistochemical detection of different levels of cervical squamous intraepithelial neoplasia HPVL1 capsid protein expression, to investigate the prediction of cervical squamous intraepithelial neoplasia Change to the value of development.