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目的探讨黄芪、党参治疗心力衰竭的可能作用机制。方法 48只C57BL/6J小鼠随机分为假手术组、模型组、黄芪组、党参组,每组12只。除假手术组外其余各组采用缩窄胸主动脉的方法建立心力衰竭小鼠模型,3天后黄芪组给予黄芪颗粒制剂12 g/(kg·d),党参组给予党参颗粒制剂6 g/(kg·d),假手术组和模型组给予生理盐水20 ml/(kg·d),各组均灌胃4周。检测各组小鼠心功能,收缩功能指标包括射血分数(EF)、左室短轴收缩率(FS),舒张功能指标包括二尖瓣环口处的血流早期峰波(E)、晚期充盈波(Ea),并计算E/Ea;分离心肌细胞,通过激光共聚焦显微镜记录钙瞬变的峰值、回落时间。结果与假手术组比较,模型组EF、FS、Ea均明显下降,而E/Ea明显升高,钙瞬变峰值下降,回落时间延长(P<0.01)。与模型组比较,黄芪组EF、FS、E、Ea升高,E/Ea降低,钙瞬变回落时间缩短;党参组EF、FS、钙瞬变峰值升高,钙瞬变回落时间缩短(P<0.05或P<0.01)。结论党参、黄芪能缩短心力衰竭小鼠心肌细胞钙瞬变回落时间,党参能提高其钙瞬变峰值,可能是其治疗心力衰竭作用机制之一。
Objective To investigate the possible mechanism of astragalus and Codonopsis in the treatment of heart failure. Methods 48 C57BL / 6J mice were randomly divided into sham operation group, model group, Astragalus membranaceus group and Codonopsis group, with 12 rats in each group. In addition to the sham operation group, the mice in the heart failure model were established by narrowing the thoracic aorta. Astragalus membranaceus was given 12 g / (kg · d) kg · d), sham operation group and model group were given normal saline 20 ml / (kg · d), each group were gavage for 4 weeks. Cardiac function was measured in each group. The indexes of systolic function included ejection fraction (EF), left ventricular short axis systolic (FS), diastolic function including early peak wave (E) of blood flow at mitral annulus, Ea was filled and E / Ea was calculated. Cardiomyocytes were isolated and the peak and fall time of calcium transient were recorded by laser scanning confocal microscopy. Results Compared with the sham operation group, the EF, FS and Ea in the model group decreased significantly while the E / Ea increased significantly. The peak of calcium transient decreased and the fall time prolonged (P <0.01). Compared with the model group, the EF, FS, E, Ea and E / Ea of Astragalus membranaceus group decreased and the transient decline time of calcium transients was shortened. The peaks of EF, FS and Ca2 + transients of CGRP group were shortened <0.05 or P <0.01). Conclusion Radix Codonopsis and Radix Astragali can shorten the time of calcium transients in cardiomyocytes of heart failure mice. Codonopsis can increase the peak of calcium transient, which may be one of its mechanisms of treating heart failure.