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本文报告应用酶联与放射免疫计量试验(IEMA与IRMA)对急性感染发热和慢性无症状两型患者血清内肠相关抗原的单种游离表位进行常规检测和读数分析。慢性感染血样采自江西玉山县,经粪便标准孵化法计数毛蚴,吡喹酮化疗后三个月再次取血,一年粪便复查;急性感染血样由湖北应城市、江陵县两血吸虫病防治站验证提供:健康对照血样采自本校学生,经环卵和血凝验证均阴性者留用。 试验所用的两株单抗(M-54,M-120)均属IgG_1
This article reports the use of enzyme-linked immunosorbent assay and radioimmunoassay (IEMA and IRMA) routine detection and reading analysis of single free epitopes of serum-gut-associated antigens in both acute and chronic asymptomatic patients. Chronic infection of blood samples collected from Yushan County, Jiangxi Province, standard hatching count by count of miracidia, praziquantel three months after the reabsorption of blood, a year review of the stool; acute infection of blood samples from Yingcheng City, Hubei Province, Jiangling County, two schistosomiasis control station verification Provide: healthy control blood samples taken from our school students, confirmed by the ring eggs and blood coagulation were negative for retention. The two monoclonal antibodies (M-54, M-120) used in this experiment are IgG1