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氯化卞法微量提取猪瘟病菌7个生理小种和马唐瘟病菌基因组DNA,用60个随机引物进行PCR扩增,其中21个引物有特异性扩增荣带,每一引物─菌系组合扩增到3~19条,平均10.3条;其中有多态性条带为1~12条,平均6.1条,多态性为61.1%。说明稻瘟病菌群体有丰富的RAPD多态性。引物OPH1,OPH2,OPH4,OPH13,OPH14,OPK14等扩增的条带多,多态性丰富,而且可以有效地区分稻瘟病菌小种间的差异。几乎所有21个引物都能区别稻瘟病菌与马唐瘟病菌间的差异,而且有些引物如OPG3,OPG8,OPG13,OPK1等所扩增的产物表现为,稻瘟病菌生理小种间条带少,多态性差,而马唐瘟的条带多。说明两者在遗传上存在较大差异。
Chloropyrondemic method was used to extract genomic DNA of seven physiological races and Bradyrhizobium oryzae pathogenic bacteria in a small amount. Sixty random primers were used to amplify the genomic DNA. Among them, 21 primers were used to amplify the specific bands. Each primer- The number of polymorphic bands ranged from 1 to 12 with an average of 6.1 bands and the polymorphism was 61.1%. This shows that the rice blast germplasm is rich in RAPD polymorphism. Primer OPH1, OPH2, OPH4, OPH13, OPH14, OPK14 and other amplified bands, rich in polymorphism, and can effectively distinguish between the races of Magnaporthe grisea interspecific differences. Almost all of the 21 primers can distinguish between Magnaporthe grisea and M. cuneatum, and the products amplified by some primers such as OPG3, OPG8, OPG13 and OPK1 showed less bands between physiological races of Magnaporthe grisea , Polymorphism is poor, and more horses plague strip. Shows that there is a big difference between the two genetics.