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采用溶液培养研究了以硝酸铵和尿素作为氮源时，缺镍对水稻植株的生长、脲酶和谷氨酸胺合成酶活性、植株中尿素态N和总氮含量的影响。结果表明，以硝酸铵作为氮源时，缺镍不影响植株的生长;但以尿素作为氮源时植株生长受到明显影响，植株生长停滞，叶片黄化，叶尖坏死干枯。缺镍植株中脲酶活性非常低，地上部仅为供镍植株的0.05％左右;谷氨酰胺合成酶活性除供应尿素氮缺镍植株较低以外，其它处理没有明显差异。缺镍植株中尿素态N含量明显高于供镍植株，特别是供应尿素态氮缺镍植株地上部尿素态N大量累积。供应尿素态氮缺镍植株含氮量明显低于其它处理。
The effects of nickel deficiency on rice plant growth, urease and glutamine synthetase activities, urea N and total nitrogen in plants were studied by solution culture using ammonium nitrate and urea as nitrogen sources. The results showed that when ammonium nitrate was used as nitrogen source, the lack of nickel did not affect the growth of the plants. However, the growth of the plants was obviously affected by urea as the nitrogen source. The growth of the plants was stagnant, the leaves were yellow and the necrotic leaves withered. The urease activity in nickel-deficient plants was very low, and the shoots were only about 0.05% of that for nickel plants. There was no significant difference in glutamine synthetase activity except for the supply of urea-nitrogen deficient-nickel plants. The content of urea N in the nickel-deficient plants was significantly higher than that of the nickel-supplying plants, especially the accumulation of urea N in the upper part of the plants deficient in supply of urea nitrogen. Supply of nitrogen-deficient urea nitrogen plants nitrogen content was significantly lower than other treatments.