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为了优选土茯苓多糖的脱色材料以及获得其最佳脱色工艺参数,以脱色率和多糖保留率为考察指标,通过静态吸附法从6种不同的树脂中优选出脱色效果最佳的树脂;随后通过单因素实验考察其上样量、脱色p H、脱色温度、脱色时间、脱色转速分别对脱色率、多糖保留率的影响。在单因素的基础上,采用四因素三水平的Box-Behnken实验设计,建立并分析了各因素分别与脱色率、多糖保留率之间的数学模型。结果表明:大孔树脂XAD-16对土茯苓多糖脱色效果最佳,其最优工艺参数为:上样量6 m L、时间1.8 h、温度47.02℃、转速110 r/min。对此优化条件进行验证,脱色率为80.07%,多糖保留率为73.52%,其相对误差分别为1.54%和2.76%。土茯苓多糖通过XAD-16型树脂脱色获得了良好的色泽,并且在除去蛋白质的同时未改变多糖的溶解性。该响应面分析模型具有较好的预测性能,得到的参数条件准确可靠,可用于指导生产实践。
In order to optimize the decolorization material of Smilax glabra and to obtain the best decolorization process parameters, taking the decolorization rate and polysaccharide retention rate as indexes, the best decoloring resin was selected from 6 different resins by static adsorption method; The influence of the amount of sample, decolorization p H, decolorization temperature, decolorization time and decolorization speed on decolorization rate and polysaccharide retention rate were investigated by single factor experiment. On the basis of single factor, the four-factor and three-level Box-Behnken experimental design was used to establish and analyze the mathematical model of each factor separately with the decolorization rate and polysaccharide retention rate. The results showed that macroporous resin XAD-16 had the best decolorization effect, and the optimum technological parameters were as follows: loading amount 6 m L, time 1.8 h, temperature 47.02 ℃, speed 110 r / min. The optimized conditions were verified, the decolorization rate was 80.07%, polysaccharide retention rate was 73.52%, and the relative errors were 1.54% and 2.76% respectively. Smilax Polysaccharide obtained a good color by decolorization of XAD-16 type resin, and did not change the solubility of the polysaccharide while removing the protein. The response surface analysis model has good predictive performance, the parameters obtained are accurate and reliable, and can be used to guide the production practice.