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采用AFLP分子标记技术,以中国东海日本鳗鲡2个群体(闽江流域玻璃鳗养成的成鳗和长江口捕获的玻璃鳗)为材料,研究其遗传多样性水平及群体的遗传结构。结果表明,6对选择性扩增引物共检测到363个位点,其中闽江流域群体和长江口群体的多态位点数、多态位点频率、Nei′s基因多样性系数、Shannon′s信息指数分别为228和269、62.81%和74.10%、0.2781和0.3077、0.4092和0.4493,日本鳗鲡这2个群体均具有较丰富的遗传变异,但闽江流域群体明显低于长江口群体。2群体的遗传相似度为0.8140,遗传距离为0.2103,在用Nei′s遗传距离构建的遗传聚类图上,2群体明显分为2支,显示了群体分化现象,不同地理群到达产卵地的时间不同可能是造成这种现象的主要原因。
AFLP molecular markers were used to study the genetic diversity and population genetic structure of two populations of Japanese eel (Anguilla anguilla) from the East China Sea (the eel from the glass mullet in the Minjiang valley and the glass eel captured from the Changjiang estuary). The results showed that 363 loci were detected by 6 pairs of selective amplification primers. Among them, the polymorphic loci, polymorphic loci, Nei’s gene diversity coefficient, Shannon’s The information index was 228 and 269, 62.81% and 74.10%, 0.2781 and 0.3077, 0.4092 and 0.4493 respectively. The two populations of Japanese eel, Anguilla japonica, had rich genetic variation, but the population of Minjiang basin was significantly lower than that of Yangtze estuary. The genetic similarity between two groups was 0.8140 and the genetic distance was 0.2103. On the genetic cluster map constructed with Nei’s genetic distance, the two groups were obviously divided into two groups, which showed the phenomenon of population differentiation and different geographic groups reached the spawning ground The different time may be the main reason for this phenomenon.