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为探讨镉染毒和染毒中止后成骨细胞增殖、分化及矿化等生物学功能的改变,研究人员采用混合酶消化法分离Sprague Dawley大鼠颅盖骨成骨细胞,在5%CO2、37℃条件下培养24 h后,持续镉染毒组以不同浓度的氯化镉
To investigate the biological function of osteoblast proliferation, differentiation and mineralization after cadmium exposure and exposure, we isolated calvarial osteoblasts from Sprague Dawley rats by mixed enzyme digestion and cultured in 5% CO2, After incubation at 37 ℃ for 24 h, cadmium chloride was continuously exposed to cadmium at different concentrations