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目的明确贵州省黔西南州贞丰县百香果炭疽病的致病病原菌，并筛选出防治百香果炭疽病的有效杀菌剂。方法采用组织块分离法对发病果实进行分离和纯化，并进行柯赫氏法则验证，结合形态学和多基因分子系统学进行鉴定，同时采用菌丝生长速率法研究6种杀菌剂对该病原菌菌丝生长的抑制作用。结果引起贵州省黔西南州贞丰县百香果炭疽病的病原菌为果生刺盘孢Colletotrichum fructicola；室内毒力测定发现6种杀菌剂对该病原菌菌丝生长均有一定的抑制作用，其中10%苯醚甲环唑WG的抑制效果最好，EC50为0.557 9 mg·L−1，其次是75%肟菌·戊唑醇WG、18.7%丙环·嘧菌酯SE和35%氟菌·戊唑醇SC，其EC50分别为1.035 4、2.772 6、2.841 5 mg·L−1。结论明确了贞丰县百香果炭疽病的致病菌为果生刺盘孢；10%苯醚甲环唑WG、75%肟菌·戊唑醇WG、18.7%丙环·嘧菌酯SE和35%氟菌·戊唑醇SC对该菌具有较好的抑制作用，可作为防治百香果炭疽病的化学杀菌剂。“,”ObjectiveTo identify the pathogen of anthracnose and select appropriate fungicide(s) for controlling the disease on passion fruits in Zhenfeng County, Guizhou Province.MethodTissues from the diseased fruits were separated, and microorganisms isolated, purified, and identified by the Koch protocol. Morphological examinations and multi-gene (ITS, GAPDH, ACT, CHS-1) phylogeny analysis were conducted to verify the pathogen identification. Inhibitory effect of 6 fungicides on the isolated pathogen was determined based on the mycelial growth on a culture medium.ResultThe pathogen that caused anthracnose on passion fruits was identified as Colletotrichum fructicola. The in vitro toxicity test for the 6 fungicides showed that, among the fungicides, 10% difenoconazole WG exhibited the greatest lethality on the pathogen with an EC50 of 0.557 9 mg/L, followed by 75% trifloxystrobin-tebuconazole WG with an EC50 of 1.035 4 mg/L, 18.7% procyclidine-azoxystrobin SE with a EC50 of 2.7726mg/L, and 35% fluopyram-tebuconazole SC with an EC50 of 2.841 5 mg/L.ConclusionThe pathogen of anthracnose on passion fruits in the county was identified as C. fructicola. Difenoconazole, trifloxystrobin-tebuconazole, procyclidine-azoxystrobin, and fluopyram-tebuconazole were expected to have varying degrees of efficacies for field control on the disease.