目的应用高效液相色谱法对盐酸环丙沙星进行含量测定。方法选用十八烷基硅烷键合硅胶柱为色谱柱,以0.05 mol/ml枸橼酸溶液-乙腈(82∶18)为流动相,检测波长为277 nm,流速为1.00 ml/min。结果该方法的线性范围为10.12～60.72 ug/ml,方法回收率为99.9%,RSD为0.32%。结论该方法简便、灵敏、准确地对盐酸环丙沙星进行含量测定。 Objective To determine the content of ciprofloxacin hydrochloride by high performance liquid chromatography. Methods The octadecylsilane bonded silica gel column was used as the mobile phase. The detection wavelength was 277 nm and the flow rate was 1.00 ml / min with 0.05 mol / ml citric acid solution - acetonitrile (82:18). Results The linear range of this method was 10.12 ~ 60.72 ug / ml, the recovery rate was 99.9% and the RSD was 0.32%. Conclusion The method is simple, sensitive and accurate determination of ciprofloxacin hydrochloride.