论文部分内容阅读
目的:建立重庆特有药用植物缙云黄芩HPLC指纹图谱,对其根、茎、叶中4种主要的黄酮类活性成分进行分离、含量测定和比较研究。方法:采用梯度洗脱方法进行色谱分离和含量测定,记录10个种群缙云黄芩指纹图谱,采用国家药典委“中药色谱指纹图谱相似度评价系统2004A版”建立指纹图谱共有模式。结果:建立了同时分离和测定4种活性成分的梯度洗脱程序;缙云黄芩指纹图谱14个共有峰分离度良好、保留时间稳定、重现性好;供试样品间相似度较高;4种活性成分总量多少依次为根>茎>叶,尤其是黄芩苷在根中的积累量达茎中的5倍以上。结论:本研究建立的色谱指纹图谱分析方法稳定、可靠,能为缙云黄芩种源的宏观分析、探明其遗传变异模式及其保护和合理开发利用等提供重要的理论依据。
OBJECTIVE: To establish the HPLC fingerprint of the medicinal plant Jinyun skullcap in Chongqing and to isolate, determine and compare the four major flavonoid active ingredients in root, stem and leaf. Methods: The gradient elution method was used for the chromatographic separation and content determination. The fingerprints of 10 populations of Jinyun baicalin were recorded and the common pattern of fingerprints was established by the State Pharmacopoeia Commission “2004A Version of Chinese Medicines Chromatographic Fingerprint Similarity Evaluation System”. Results: A gradient elution program was established for the simultaneous separation and determination of four active ingredients. The 14 common peaks of the fingerprints of S.yunnanensis showed good resolution, good retention time and good reproducibility. Four samples The total amount of active ingredients followed by root> stem> leaf, especially baicalin accumulation in the roots of more than 5 times the stem. Conclusion: The chromatographic fingerprinting method established in this study is stable and reliable. It can provide a macroscopical analysis of the provenance of Scutellaria baicalensis Georgi and provide an important theoretical basis for its genetic variation patterns and its protection and rational development and utilization.