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为进一步了解乙型肝炎病人血白细胞内HBV抗原的存在情况,我们于1984~1986年对32例乙型肝炎病人进行了检测,现将结果报告如下。32例中临床明确诊断为急性肝炎者10例,慢活肝9例,慢迁肝6例,肝硬化1例,未分型6例。正常献血员10例。一、血浆与外周血白细胞裂解液HBsAg检测结果:采用RPHA法。血白细胞悬液RPHA滴度1:2以上29例,占90.6%(见附表)。3例治疗后血浆RPHA1:2阴性,而其白细胞悬液为1:8。2例白细胞RPHA滴度1:16以上标本,以马抗HBsAg血清作用可阻断其特异性。二、外周血白细胞中HBsAg检测结果:采
To further understand the presence of HBV antigens in leukocytes of hepatitis B patients, we tested 32 patients with hepatitis B from 1984 to 1986 and the results are reported below. Of the 32 cases, 10 cases were diagnosed as acute hepatitis, 9 cases were slow-activating liver, 6 cases were slow-moving liver, 1 case was cirrhosis and 6 cases were non-typed. Normal blood donors in 10 cases. First, the plasma and peripheral blood leukocyte lysate HBsAg test results: The RPHA method. Blood leukocyte suspension RPHA titer 1: 2 more than 29 cases, accounting for 90.6% (see table). Plasma RPHA1: 2 was negative in 3 cases, while white blood cell suspension was 1: 8.2 in white blood cells. The titer of RPHA was 1:16, and its specificity was blocked by horse anti-HBsAg serum. Second, the peripheral blood leukocytes HBsAg test results: mining