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目的:建立高效液相色谱法测定牛黄至宝丸中胆红素含量的方法。方法:色谱柱为Dikma C_(18)(250mm×4.6mm, 5μm);流动相为氯仿-甲醇-2%二乙胺水溶液(80:19:1),检测波长:450nm,流速:1 ml·min~(-1)柱温:45℃。结果:胆红素在0.63～1.89μg·ml~(-1)浓度范围内线性关系良好(r=0.999 9),加样回收率为99.4%,RSD为0.5%(n=9)。结论:本方法准确可靠,方法简便,可用于测定牛黄至宝丸中胆红素的含量。
Objective: To establish a HPLC method for the determination of bilirubin in Bezoar Zibao pill. METHODS: The chromatographic column was Dikma C 18 (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of chloroform-methanol-2% diethylamine (80:19: 1) min ~ (-1) Column temperature: 45 ℃. Results: The bilirubin showed a good linearity (r = 0.999 9) in the range of 0.63-1.89 μg · ml -1 with a recovery of 99.4% and RSD of 0.5% (n = 9). Conclusion: The method is accurate and reliable, the method is simple and can be used to determine the content of bilirubin in bezoar-to-bao pill.