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分析了高温胁迫条件下豇豆[Vigna unguiculata(L.)Walpers]幼苗叶片细胞膜透性和保护酶活性等理化指标的变化及品种间的差异,分析了高温胁迫对豇豆叶片蛋白质表达的影响.结果显示,在高温胁迫下,8个豇豆品种的热害指数差异有统计学意义,直观表现为之豇28-2和白籽豇豆耐热性强,而高产四号、宁豇三号和红豇豆的耐热性差;所有豇豆品种的细胞膜透性和丙二醛(MDA)质量摩尔浓度均有不同程度的增大,但耐热性强的品种增幅较小;超氧化物歧化酶(SOD),过氧化物酶(POD)和过氧化氢酶(CAT)活性呈现出先上升后下降的变化趋势,而耐热性强的品种增幅较大;耐热豇豆品种的可溶性糖浓度及高温胁迫后的增值均低于耐热性较差的品种;双向电泳结果表明,高温胁迫特异性诱导了15种和抑制了6种蛋白质表达,另鉴别到8种上调和10种下调表达蛋白.表明高温胁迫引起一系列生理生化和基因表达的变化.该研究为今后解析豇豆的耐热机理、选育耐热品种和发掘重要抗热基因等奠定了基础.
The changes of cell membrane permeability and activities of protective enzymes in the leaves of [Vigna unguiculata (L.) Walpers] seedlings under high temperature stress were analyzed. The effects of high temperature stress on the protein expression of cowpea leaves were analyzed. , Under the high temperature stress, the differences of the heat-damp index between the eight varieties of cowpea were statistically significant, the visual performance was that the cowpea 28-2 and the white-seed cowpea had the strong heat-tolerance, while the high-yielding No.4, Ning-wei-3 and red cowpea All the cowpea cultivars had different cell membrane permeability and malonyldialdehyde (MDA) mass concentration, but the heat-tolerant varieties had less increase. Superoxide dismutase (SOD) The activity of POD and CAT showed the trend of first rising and then decreasing, while the varieties with higher heat tolerance increased more. The soluble sugar concentration of heat-resistant cowpea varieties and the added value after high temperature stress Lower than the heat-resistant varieties; two-dimensional electrophoresis results showed that high temperature stress induced specific 15 and inhibited the expression of six proteins, and identified eight kinds of down-regulated and down-regulated expression of 10 proteins, indicating that high-temperature stress caused a series Physiological and biochemical Changes in gene expression. This study lays a foundation for the heat Analysizing cowpea breeding resistant varieties and heat-resistant genes important to explore the future.