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由禾谷多粘菌(Polymyxagraminis)传播的线状小麦花叶病毒有两种,一种是加拿大首先报道的小麦梭条斑花叶病毒(WSSMV),另一种是日本报道的小麦黄花叶病毒(WYMV)。这两种病毒粒子形态以及血清学性质非常相似,但核酸序列存在一定差异。经反转录聚合酶链反应(RTPCR)和单链构象多态性分析(SSCP),明确我国广泛发生的禾谷多粘菌传线状小麦花叶病毒都是小麦黄花叶病毒,但供试的8个分离物RNA1和RNA2序列存在差异,无一彼此完全相同
There are two linear wheat mosaic virus transmitted by Polymyxagraminis, one is wheat first leaf spot blotch virus (WSSMV) reported in Canada and the other is wheat yellow mosaic virus (WYMV). The two virus particles morphology and serological properties are very similar, but the nucleic acid sequence there are some differences. Through reverse transcription-polymerase chain reaction (RTPCR) and single-strand conformation polymorphism analysis (SSCP), it is clear that the Polyporus glutinosa cerealum transmission line wheat mosaic virus that is widely occurring in our country is wheat yellow mosaic virus, However, there were differences in the sequences of RNA1 and RNA2 among the eight isolates tested, none of which was exactly the same as each other