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目的:建立以高效液相色谱法快速简便测定六合维生素丸中维生素A含量的方法。方法:采用安捷伦TC-C18柱(150mm×4.6 mm,5μm),以甲醇-水(95∶5)为流动相,流速为1.0 ml.min-1,检测波长为328 nm。结果:维生素A在2.5~101.8μg.ml-1浓度范围内呈良好的线性关系,r=1.000 0,平均回收率为99.20%,RSD=0.82%。结论:该法专属性强,操作快速简便,结果准确。
Objective: To establish a rapid and simple method for determination of vitamin A in Liuhe vitamin pills by high performance liquid chromatography. Methods: An Agilent TC-C18 column (150 mm × 4.6 mm, 5 μm) was used with a mobile phase of methanol-water (95: 5) at a flow rate of 1.0 ml.min-1 and a detection wavelength of 328 nm. Results: There was a good linear relationship between vitamin A concentration in the range of 2.5 ~ 101.8μg.ml-1, r = 1.000 0, the average recovery was 99.20% and RSD was 0.82%. Conclusion: The method is highly specific, quick and easy to operate, the result is accurate.