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本研究目的是为了了解耵聍中有机氯农药量与体脂中农药量的关系,这关系着能否以此种生物物质做为监测人体负荷农药水平的一种材料。1.首先建立了测定耵聍中有机氯农药及脂肪含量的微量方法,样品用量为10毫克左右。五次回收试验平均回收率为:α-666 90.3,β-666 84.4,γ-666 95.4,δ-666 94.4,PP′-DDE 88.2,OP′-DDT 91.7,PP′-DDD86.0,PP′-DDT 98.3。2.正常健康男女51人,耵聍脂肪含量为8.36-43.20%,个体差异较大,因此耵聍及脂肪组织中农药含量皆以脂肪为基础计算结果。其耵脂中666、DDT含量虽因膳食中农药水平、性别、年龄不同而异,但β-666/总666及PP′-DDE/总DDT比值%变动不大。前者为62.07-71.23%,后者为63.04—73.65%。在耵聍中666各异构体含量顺序为β>α>γ>δ;DDT类则为PP′-DDE>PP′-DDT;很少检出OP′-DDT及PP′-DDD。3.78例耵脂中β-666与体脂中β-666相关系数为0.771,两者有非常显著相关。4.44例耵脂中PP′-DDE与体脂PP′-DDE相关系数为0.670,两者也有非常显著相关。本研究结果为在正常人群中监测机体受有机氯农药污染程度提供了新指标。
The purpose of this study is to understand the relationship between the amount of organochlorine pesticides and the amount of pesticides in body fat in catfish, which is related to whether such biological substances can be used as a material for monitoring the level of pesticides loaded on the human body. 1. Firstly, a trace method for the determination of organochlorine pesticides and fat content in 耵 聍 was established. The dosage of the sample was about 10 mg. The average recoveries of five recovery experiments were: α-666 90.3, β-666 84.4, γ-666 95.4, δ-666 94.4, PP’-DDE 88.2, OP’-DDT 91.7, PP’-DDD86.0, PP ’ -DDT 98.3.2. There are 51 normal and healthy men and women with a fat content of 8.36-43.20% in Emu Babies. The individual differences are large, so the contents of pesticides in Babylonica and fat tissues are calculated on the basis of fat. Although the content of DDT in fat-free fat 666 varies with the level of pesticide in the diet, the sex, age, the ratio of β-666 / total 666 and PP’-DDE / total DDT varies little. The former is 62.07-71.23%, the latter is 63.04-73.65%. The order of the isomers of 666 is β> α> γ> δ in 耵 聍, and PP’-DDE> PP’-DDT in the DDT class. OP’-DDT and PP’-DDD are seldom detected. The correlation coefficient of β-666 in 3.78 cases of fat and β-666 in body fat was 0.771, there was a very significant correlation between them. The correlation coefficient of PP’-DDE and body fat PP’-DDE in 4.44 cases of fat was 0.670, and there was also a very significant correlation between them. The results of this study provide new indicators for monitoring the extent of organochlorine pesticide contamination in the general population.