立体选择性酰胺酶是一种重要的手性合成工具酶,在制备手性羧酸及其衍生物方面具有广阔的应用前景,日益受到重视。在酰胺酶的应用中,其立体选择性影响巨大。从底物、反应温度、pH、添加共溶剂和微生物来源5个方面综述了其对酰胺酶立体选择性的影响,对提高酰胺酶的立体选择性,扩大其在制备光学活性化合物领域的应用具有重要的意义。 Stereoselective amidase is an important enzyme in chiral synthesis, which has broad application prospects in the preparation of chiral carboxylic acids and their derivatives. In the application of amidase, its stereoselectivity has a huge impact. The stereoselectivity of amidase was reviewed in terms of substrate, reaction temperature, pH, co-solvent and microorganism sources. It is of great importance to improve the stereoselectivity of amidase and to expand its application in the field of preparing optically active compounds Significance.