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我国新疆皮肤利什曼病病原体（ＣＬＰ）种株鉴定尚有分歧。本研究用一对利什曼原虫属特异引物Ｒ２２２和Ｒ３３３，直接从皮肤利什曼病病人皮肤病变组织、热带利什曼原虫（Ｌｅｉｓｈｍａｎｉａｔｒｏｐｉｃａ）和婴儿利什曼原虫（Ｌ．ｉｎｆａｎｔｕｍ）基因组ＤＮＡ中扩增出一ＳＳＵｒＤＮＡ特异片段，克隆到ｐＧＥＭ○Ｒ－ＴＥａｓｙＶｅｃｔｏｒ上，并以双脱氧链末端终止法测序。序列分析显示扩增的ＣＬＰ、Ｌ．ｉｎｆａｎｔｕｍ和Ｌ．ｔｒｏｐｉｃａＳＳＵｒＤＮＡ序列皆为３９１ｂｐ长，Ｌ．ｔｒｏｐｉｃａ与ＣＬＰ的序列之间３８７个碱基相同，存在４个点突变；Ｌ．ｉｎｆａｎｔｕｍ与ＣＬＰ的序列之间３８３个碱基相同，存在７个点突变和２个移码突变；Ｌ．ｔｒｏｐｉｃａ与Ｌ．ｉｎｆａｎｔｕｍ的序列之间３８７个碱基相同，存在３个点突变和２个移码突变。表明扩增的ＣＬＰ、Ｌ．ｉｎｆａｎｔｕｍ和Ｌ．ｔｒｏｐｉｃａＳＳＵｒＤ－ＮＡ多变区序列高度同源，但仍存在少数点突变，或插入／缺失；该ＳＳＵｒＤＮＡ序列变异可反应种间差异；新疆皮肤利什曼病病原体与Ｌ．ｔｒｏｐｉｃａ较相近。
China’s Xinjiang skin Leishmaniasis pathogens (CLP) identification of strains are still different. In this study, a pair of Leishmania-specific primers, R222 and R333, were used directly in the skin lesions of cutaneous leishmaniasis patients, Leishmaniatropica and L.infantum genomic DNA A specific SSUrDNA fragment was amplified, cloned into pGEM® R-TEasyVector and sequenced by dideoxy chain termination. Sequence analysis revealed amplified CLP, L. infantum and L. TropicaSSUrDNA sequences are 391bp long, L. 387 bases between the tropica and CLP sequences are the same, there are 4 point mutations; The 383 bases between the infantum and CLP sequences are the same, there are seven point mutations and two frameshift mutations; tropica and L. The sequence of infantum is 387 bases in length with three point mutations and two frameshift mutations. Expressed CLP, L. infantum and L. tropic SSUrD-NA variable region sequence is highly homologous, but there are still a few point mutations, or insert / deletion; the SSUrDNA sequence variation can reflect differences between species; Xinjiang skin leishmaniasis pathogen and L. Tropica is more similar.