按照Fauci等方法,取100微升压积红细胞加入1毫升现配的三氯化铬(100微克/ml盐水)和100微升葡萄球菌蛋白A(1.25毫升/ml)中。在30℃孵育40分钟后用盐水离心细胞洗涤两次。用含1毫克/ml牛血清白蛋白的PBS配制成2.25×10~8/ml的葡萄球菌蛋白A致敏羊红细胞悬液(E-SPA)。在微量滴定板上,用含10％胎牛血清(FCS)的RPMI 2倍稀释从正常小鼠血清提取的免疫球蛋白(IgG和 According to the method of Fauci et al., 100 microliters of hematocrit were added to 1 ml of chromium trichloride (100 micrograms / ml saline) and 100 microliters of staphylococcal protein A (1.25 ml / ml). After incubation for 40 minutes at 30 ° C, the cells were washed twice with saline and centrifuged. Staphylococcal protein A-sensitized sheep erythrocyte suspension (E-SPA) was formulated in PBS containing 1 mg / ml bovine serum albumin at 2.25 x 10 ~ 8 / ml. On microtiter plates, immunoglobulins extracted from normal mouse sera were immunoblocated with RPMI containing 10% fetal bovine serum (FCS) (IgG and