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扁桃酸酰胺(Mandelic Amide)已证实为桃叶膏抗滴虫的有效成份,为探讨此成份的杀虫机制,作者曾作了光镜下的形态观察,而对电镜下的超微结构改变未见有过记载。本文就电镜下的观察结果报导如下。观察方法是在培养48小时的滴虫培养管内加入40mg/5ml扁桃酸酰胺,培养48小时后制备样品。另外取一滴虫培养管不加药品作对照组,并与实验管同时进行样品制备。样品制备是将上述两种标本,先以2.5％戌二醛液在室温下作一级固定,15分钟后,用pH7.4磷酸缓冲液水洗1小时,再入1％四氧化锇二级固定2小时。用乙醇进行系列脱水,用环氧树酯包埋处理48小时,作超薄切片,切片用醋酸铀和柠檬铅作双染色,随后在H—600型电镜下观察拍片。
Mandelic Amide has been proved to be an effective component of Peach Trichomonas Trichomonas. In order to explore the insecticidal mechanism of this component, the author made morphological observation under light microscope, while the ultrastructural changes under electron microscope were not observed See have recorded. This article reports the results under electron microscopy as follows. The observation method was to add 40 mg / 5 ml of mandelic acid amide in a trichomonad culture tube cultured for 48 hours and prepare samples after culturing for 48 hours. In addition to take a drop of worm culture tube without drugs for the control group, and the experimental tube at the same time the sample preparation. Sample preparation is the above two specimens, first with 2.5% carbid dialdehyde solution at room temperature for a fixed after 15 minutes, pH7.4 phosphate buffer washed 1 hour, then re-1% osmium tetroxide secondary fixation 2 hours. A series of dehydration with ethanol, embedded in epoxy resin for 48 hours, as ultra-thin sections, sections with uranyl acetate and lemon lead for double staining, followed by observation under H-600 electron microscope filming.