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目的探讨组蛋白去乙酰化酶2(HDAC2)在食管鳞癌组织中的表达及其临床意义。方法应用实时荧光定量PCR及Western blot法检测30例配对人食管鳞癌(A组)和癌旁组织(C组)中HDAC2蛋白及其mRNA的表达。免疫组化方法检测90例(B组)食管鳞癌组织中HDAC2的表达水平,分析其与临床和病理特性的相关性。结果 A组HDAC2蛋白及其mRNA表达量均显著高于C组(P<0.01)。免疫组化结果显示,B组HDAC2蛋白表达阳性率为79%(71/90),HDAC2表达强度与TNM分期和淋巴结转移相关(P<0.05),而与患者年龄、性别、肿瘤大小、肿瘤位置、病理学大体分型、浸润深度及分化程度无关(P>0.05)。结论 HDAC2高表达可能是食管鳞癌的特征之一,在一定程度上可以反映肿瘤的恶性程度,可作为判断食管鳞癌患者预后的指标。
Objective To investigate the expression of histone deacetylase 2 (HDAC2) in esophageal squamous cell carcinoma and its clinical significance. Methods The expression of HDAC2 protein and its mRNA in 30 paired human esophageal squamous cell carcinomas (group A) and adjacent noncancerous tissues (group C) were detected by real-time fluorescence quantitative PCR and Western blot. The expression of HDAC2 in 90 cases (group B) esophageal squamous cell carcinoma was detected by immunohistochemistry, and its correlation with clinical and pathological features was analyzed. Results The expression of HDAC2 protein and mRNA in group A were significantly higher than those in group C (P <0.01). Immunohistochemistry showed that the positive expression rate of HDAC2 protein in group B was 79% (71/90), the expression of HDAC2 was correlated with TNM stage and lymph node metastasis (P <0.05), but not with age, gender, tumor size, tumor location , Pathological general classification, depth of invasion and the degree of differentiation (P> 0.05). Conclusion High expression of HDAC2 may be one of the characteristics of esophageal squamous cell carcinoma, to a certain extent, it can reflect the malignant degree of the tumor and can be used as an index to judge the prognosis of esophageal squamous cell carcinoma.