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对中国南方海域的青石斑鱼(Epinephelus awoara)、赤点石斑鱼(E.akaara)、橙点石斑鱼(E.bleekeri)及蜂巢石斑鱼(E.merra)进行了遗传多样性和分子系统发生关系的分析。对9个微卫星DNA标记的基因型测定结果进行计算,发现这4个地理分布重叠且形态相似的石斑鱼均具有较丰富的遗传多样性,其平均等位基因数、平均多态信息含量(PIC)、平均观测杂合度(Ho)及平均期望杂合度(He)的取值范围分别为(8.22±5.02)～(18.67±9.38)、(0.56±0.21)～(0.83±0.13)、(0.62±0.03)～(0.85±0.02)和(0.60±0.07)～(0.86±0.04)。经哈迪-温伯格平衡(HWE)检验,除橙点石斑鱼外,其他3种鱼的微卫星DNA位点都基本符合平衡。利用微卫星DNA标记和线粒体细胞色素b基因对这4种鱼重建的分子系统树均显示,青石斑鱼与赤点石斑鱼的亲缘关系较近。以微卫星DNA标记对所研究石斑鱼的全部个体进行贝叶斯聚类分析的结果表明,4个鱼物种之间的分化关系明确。
Epinephelus awoara, E.akaara, E.bleekeri and E. merra in the South China Sea were studied for genetic diversity and genetic diversity. Analysis of the Relationship between Molecular Systems. The results of nine microsatellite DNA genotypes were calculated and found that the four geographically distributed and similar groupers all had rich genetic diversity with the average number of alleles and the average polymorphic information content (PIC), mean observed heterozygosity (Ho) and mean expected heterozygosity (He) ranged from 8.22 ± 5.02 to 18.67 ± 9.38 and from 0.56 ± 0.21 to 0.83 ± 0.13, respectively 0.62 ± 0.03) ~ (0.85 ± 0.02) and (0.60 ± 0.07) ~ (0.86 ± 0.04), respectively. The Hardy-Weinberrian equilibrium (HWE) test showed that the microsatellite DNA sites of the other three species of fish were basically in balance with the exception of the orange-spotted grouper. Molecular phylogenetic tree reconstruction using microsatellite DNA markers and mitochondrial cytochrome b genes showed that the green grouper was closely related to the red grouper. The results of Bayesian clustering analysis of all individuals of grouper studied with microsatellite DNA markers showed that the differentiation between the four fish species was clear.