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人外周血单个核细胞(PBMC)在含高水平重组IL-2(rIL-2)的培养液中进行培养,所产生的淋巴因子活化杀伤细胞(LAK)具有广谱抗肿瘤活性。但人们对LAK细胞前体和效应细胞的表型所知甚少。用通常的培养方法,5天后LAK 细胞活性迅速下降。本文作者建立了低密度淋巴细胞的一种体外长期培养方法,能稳定地产生大量LAK 细胞,并具有较高的细胞毒活性。作者对这些细胞的表型特征也进行了探讨。首先分离PBMC,再经离心分离到大颗粒幼稚淋巴细胞。将所得细胞在含有25mM
Human peripheral blood mononuclear cells (PBMCs) are cultured in a medium containing high levels of recombinant IL-2 (rIL-2) and the produced lymphokine-activated killer cells (LAKs) have a broad spectrum of anti-tumor activity. However, little is known about the phenotype of LAK cell precursors and effector cells. With usual culture methods, LAK cell activity rapidly decreased after 5 days. The authors established an in vitro long-term culture method of low-density lymphocytes, which can stably produce large amounts of LAK cells and have high cytotoxic activity. The authors also explored the phenotypic characteristics of these cells. PBMCs were first isolated and then centrifuged to isolate large particles of naive lymphocytes. The resulting cells were grown in DMEM containing 25 mM