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为开展41Ca示踪研究破骨细胞骨吸收行为,实验采用人工合成含41Ca的羟基磷灰石骨粉(HA),制备出粒度为20～50nm的羟基磷灰石超细粉体后,经X射线衍射检验,验证其结构为羟基磷灰石。破骨细胞示踪实验在复旦大学放射医学研究所进行。在细胞培养前一天预先将含41Ca的HA细颗粒进行高压灭菌,溶解于Tc199溶液,均匀接种于96孔板的培养孔中。参照已建立的破骨细胞分离方法,从新生Wistar大鼠四肢长骨分离破骨细胞,培养于含41Ca羟基磷灰石骨粉的96孔板中。然
In order to carry out the 41Ca tracing study on the osteoclast bone resorption behavior, artificial synthesis of hydroxyapatite bone powder (HA) containing 41Ca was used to prepare the ultrafine hydroxyapatite powder with the particle size of 20-50nm. Diffraction test to verify the structure of hydroxyapatite. The osteoclast tracer experiment was conducted at the Radiation Medicine Institute of Fudan University. One day prior to cell culture, HA fine particles containing 41Ca were autoclaved and dissolved in Tc199 solution and uniformly seeded in 96-well culture plates. With reference to established osteoclast isolation methods, osteoclasts were isolated from long bones of newborn Wistar rats and cultured in 96-well plates containing 41Ca hydroxyapatite bone meal. Of course