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目的 :在双特异diabody中设计和引入二硫键结构 ,提高其稳定性。 方法 :在抗HBsAg/RBC双特异dia body表达载体中分别将抗HBsAg或抗RBC抗体的轻重链可变区基因适当部位进行突变 ,引入半胱氨酸残基 ,使diabody结构中形成共价键。大肠杆菌中分泌表达或包含体表达。 结果 :使抗HBsAg/RBC双特异diabody的热稳定性和在尿素中的稳定性得到了较大程度的提高 ,改善了diabody的性能。 结论 :证明了diabody二硫键模型的可行性
OBJECTIVE: To design and introduce disulfide bonds in bispecific diabodies to improve their stability. METHODS: The appropriate sites of the light chain and heavy chain variable region of anti-HBsAg or anti-RBC antibody were mutated respectively in anti-HBsAg / RBC bispecific diabody expression vector to introduce cysteine residues to form a covalent bond in the diabody structure . Escherichia coli secretes or contains the expression of the body. RESULTS: The thermal stability of the anti-HBsAg / RBC bispecific diabodies and their stability in urea were greatly improved, improving the diabody performance. Conclusion: The feasibility of the diabody disulfide bond model is demonstrated