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为探索花生过敏原致敏机理,采用固相合成肽技术合成Ara h1的23条多肽。以花生过敏患者血清为抗体,鉴别和定位Ara h1的抗原决定簇。结果表明:Ara h1第21～34位,第89～98位,第393～403位,第498～507位,第594～605位氨基酸序列识别率在60%以上,为Arah1的抗原决定簇,其中第498～507位的多肽识别率为100%,是显性抗原决定簇,其氨基酸序列为RRYTARLKEG。用丙氨酸依次取代显性抗原决定簇的每个氨基酸,结果抗原决定簇的致敏性增强或丧失,说明Ara h1第499位和第503位的精氨酸和第502位的丙氨酸为降低Ara h1致敏性的关键氨基酸。
In order to explore the sensitization mechanism of peanut allergen, 23 polypeptides of Ara h1 were synthesized by solid phase synthetic peptide technology. Using peanut allergic serum as an antibody, the antigenic epitopes of Arah1 were identified and mapped. The results showed that the recognition rate of amino acid sequence of Arah1 was 21 to 34, 89 to 98, 393 to 403, 498 to 507, and 594 to 605 were above 60% The first 498 ~ 507 of the polypeptide recognition rate of 100%, is a dominant epitope, the amino acid sequence is RRYTARLKEG. Substitution of each of the amino acids of the dominant epitopes with alanine in turn resulted in enhanced or loss of allergenicity of the antigenic determinant indicating that arginine at positions 499 and 503 of Ara h1 and alanine at position 502 Key amino acids that reduce Ara h1 sensitization.