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目的探讨检测人乳头瘤病毒(HPV)-DNA分型检测对35岁以下妇女宫颈病变的诊断价值,为宫颈病变的筛查提供依据。方法对2013年1-12月收治的126例35岁以下妇女采用核酸分子快速杂交基因分型技术进行HPV-DNA分型检测及阴道镜下多点病理活检,分析HPV-DNA分型在不同级别细胞学诊断中的检测结果,并与病理结果进行对比。结果 HPV-DNA分型检测阳性率为60.23%,8种最常见HPV感染频率由高至低为HPV-16、HPV-52、HPV-58、HPV-18、HPV-11、HPV-6、HPV-33、HPV-39;在细胞学诊断中,低度鳞状上皮内病变(LSIL)和高度鳞状上皮内病变(HSIL)HPV检出率(分别为83.33%、100.00%)差异无统计学意义(χ2=0.18,P>0.05);且两者均与不明确意义的非典型鳞状上皮细胞(ASC-US)细胞学诊断HPV检出率(56.25%)差异有统计学意义(P<0.05);HPV阳性病理确诊为宫颈上皮内瘤变(CIN)者47例,HPV阴性病理确诊为宫颈上皮内瘤变(CIN)者12例,差异有统计学意义(χ2=43.57,P<0.05)。结论HPV-DNA分型检测对35岁以下妇女宫颈病变的筛查具有较高的指导意义,且高危型HPV感染与宫颈病变的严重程度密切相关,能够为其病情和疗效评估提供科学依据。
Objective To investigate the diagnostic value of detecting human papillomavirus (HPV) -DNA typing in cervical lesions in women under 35 years of age, and to provide evidence for the screening of cervical lesions. Methods 126 cases of women under 35 years old admitted from January to December in 2013 were detected by HPV genotyping and colposcopy biopsy under the condition of rapid hybridization with nucleic acid. The HPV-DNA typing was analyzed at different levels Cytological diagnosis of the test results, and compared with the pathological results. Results The positive rate of HPV-DNA typing was 60.23%. The frequency of HPV-16, HPV-52, HPV-58, HPV-18, HPV-11, HPV- -33 and HPV-39 respectively. There was no significant difference in the cytological diagnosis between low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL) (83.33%, 100.00%) (Χ2 = 0.18, P> 0.05). There was a statistically significant difference between the two methods in the cytological diagnosis of atypical squamous cell (ASC-US) with undefined significance (56.25%) (P < 0.05). There were 47 cases with positive diagnosis of cervical intraepithelial neoplasia (CIN) and 12 cases with cervical intraepithelial neoplasia (CIN) with HPV negative pathology (χ2 = 43.57, P <0.05) ). Conclusion The detection of HPV-DNA typing is of high guiding significance for the screening of cervical lesions in women under 35 years of age. High-risk HPV infection is closely related to the severity of cervical lesions, which can provide a scientific basis for the evaluation of the disease and efficacy.