硒蛋氨酸是普遍存在于植物蛋白中的一种常见的硒氨基酸,它的测定对研究必需微量元素硒的营养以及硒与人体健康的关系有重要意义。 在蛋白质中,硒蛋氨酸总是与同系物蛋氨酸共存,其含量极微,在6mol/LHC1中水解7小时便完全分解,因此,氨基酸分析常用的酸水解—离子交换色谱分析法不适用于硒蛋氨酸的定量分析。Caldwell等 Selenomethionine is a common selenoamino acid which is ubiquitous in plant proteins. Its determination is of great significance for the study of the nutrition of essential trace element selenium and the relationship between selenium and human health. Among the proteins, selenomethionine always coexists with the homologue methionine, and its content is extremely low. It completely decomposes in 6mol / L HCl for 7 hours. Therefore, the acid hydrolysis-ion exchange chromatography commonly used for amino acid analysis is not suitable for selenomethionine Quantitative analysis. Caldwell et al