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观察氧化槐定碱(oxysophoridine,OSR)鞘内注射(intrathecal injection,ith)的镇痛作用并探讨与GABAA受体相关的作用机制。采用小鼠温浴法测定痛阈,观察OSR(ith)的镇痛作用及GABAA受体激动剂和拮抗剂对OSR镇痛作用的影响;采用免疫组化法检测OSR对小鼠脊髓背角GABAARα1蛋白表达的影响。结果显示,OSR(12.5和6.25 mg·kg-1,ith)能明显延长热甩尾潜伏期(P<0.05,P<0.01),痛阈提高率达68.45%。GABA和蝇蕈醇(MUS)与OSR有协同镇痛作用,印防己毒素(PTX)和荷包牡丹碱(BIC)能拮抗OSR的镇痛作用。OSR(12.5 mg·kg-1,ith)可使脊髓背角GABAARα1表达明显增多(P<0.01)。结果提示,OSR(ith)具有明显的镇痛作用,脊髓GABAA受体参与了OSR的镇痛机制。
To observe the analgesic effect of intrathecal injection (ith) with oxysophoridine (OSR) and explore the mechanism of action related to GABAA receptor. The pain threshold was measured by mouse warm bath method. The analgesic effect of OSR (ith) and the analgesic effect of GABAA receptor agonist and antagonist on OSR were observed. Immunohistochemistry was used to detect the effect of OSR on the spinal dorsal horn GABAARα1 protein The impact of expression. The results showed that OSR (12.5 and 6.25 mg · kg-1, ith) could prolong the incubation period (P <0.05, P <0.01). The improvement rate of pain threshold was 68.45%. GABA and muscimol (MUS) have a synergistic analgesic effect with OSR, and picrotoxin (PTX) and bicuculline (BIC) antagonize the analgesic effect of OSR. OSR (12.5 mg · kg-1, ith) significantly increased the expression of GABAARα1 in spinal dorsal horn (P <0.01). The results suggest that OSR (ith) has obvious analgesic effect, and GABAA receptor of spinal cord is involved in the analgesic mechanism of OSR.