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将分泌抗大鼠树突状细胞单克隆抗体的杂交瘤细胞株,接种BALb/c小鼠腹腔,取腹水用正辛酸沉淀法纯化得到单克隆抗体WZD3.应用该单抗阻断试验,探讨WZD3对树突状细胞参与的淋巴细胞转化试验及混合淋巴细胞培养的影响。实验结果显示,树突状细胞在两种反应系统中的作用受到明显抑制,其抑制程度随WZD3稀释度的增加而降低,在淋巴细胞转化试验中的最大抑制率达96.4%。
The hybridoma cell line secreting monoclonal antibody against rat dendritic cells was inoculated into the peritoneal cavity of BALB / c mice and the ascites fluid was purified by n-octanoic acid precipitation to obtain the monoclonal antibody WZD3. The monoclonal antibody blocking test was used to investigate the effect of WZD3 on dendritic cells involved in lymphocyte transformation and mixed lymphocyte culture. The experimental results show that the dendritic cells in the two reaction system was significantly inhibited the degree of inhibition with the WZD3 dilution decreased, the maximum inhibition rate in lymphocyte transformation test was 96.4%.