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取正常Ｗｉｓｔａｒ大鼠，用福氏痢疾杆菌及福氏完全佐剂免疫动物，免疫成熟后处死取材。石腊切片，ＡＢＣ法染色，ＤＡＢ显色。光镜下观察下丘脑弓状核促肾上腺皮质激素样免疫反应和β－内啡肽样免疫反应阳性细胞的形态、分布和计数。图像分析仪测其灰度值。结果提示免疫老龄鼠神经内分泌与免疫系统间的调节功能紊乱，可能是引起老龄鼠免疫力低下的原因之一。
Normal Wistar rats were used to immunize animals with Shigella flexneri and Freund’s complete adjuvant. Paraffin sections, ABC staining, DAB color. The morphology, distribution and count of adrenocorticotrophic immunoreactivity and β-endorphin-like immunoreactive cells in the arcuate nucleus of the hypothalamus were observed under light microscope. Image analyzer to measure its gray value. The results suggest that the immunological regulation of neuroendocrine and immune system immune function in aged rats may be one of the causes of poor immunity in aged mice.