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克隆获得了大豆转录因子MYB基因GmMYB174,该基因序列全长1086 bp,编码361个氨基酸,属于MYB-related家族。生物信息学分析表明大豆GmMYB174与番茄、苜蓿、葡萄等物种同源性较高,序列分析表明包含2个保守的Trp(W)位点和1个保守基序SHAQKFF。亚细胞定位结果显示GmMYB174定位于细胞核中;组织表达量显示GmMYB174在多个组织均有表达,其中在上胚轴中表达量最高。启动子元件分析表明GmMYB174包含ABRE、MYB、MYC、LTRE、GT-1等逆境胁迫应答元件。实时荧光定量PCR分析表明Gm MYB174在干旱、盐、ABA处理下均有响应。因此,GmMYB174可能参与多种胁迫应答途径。
The MYB gene GmMYB174 was cloned and the gene was 1086 bp in length and encoded 361 amino acids. It belongs to the MYB-related family. Bioinformatics analysis showed that soybean GmMYB174 shared high homology with tomato, alfalfa and grape, and the sequence analysis indicated that it contained two conserved Trp (W) sites and one conserved motif SHAQKFF. The results of subcellular localization showed that GmMYB174 localized in the nucleus. The expression of GmMYB174 in many tissues showed the highest expression in the hypocotyls. Analysis of promoter elements showed that GmMYB174 contained ABR, MYB, MYC, LTRE and GT-1 stress response elements. Real-time PCR analysis showed that Gm MYB174 responded to drought, salt and ABA treatments. Therefore, GmMYB174 may participate in a variety of stress response pathways.