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为探究解淀粉芽胞杆菌X60作为烟草青枯病生防菌剂的潜力,采用Biolog代谢表型技术比较了2种细菌的不同代谢表型。结果表明,烟草青枯病菌和解淀粉芽胞杆菌分别能代谢19%、41%的碳源,43%、77%的氮源,95%、86%的磷源以及100%、69%的硫源,分别有94、91种生物合成途径,49、95种渗透压表型以及19、94种pH代谢表型;解淀粉芽胞杆菌比烟草青枯病菌代谢显著的碳源有L-果胶糖、D-甘露糖等34种,氮源有腺苷、胞苷等29种;烟草青枯病菌比解淀粉芽胞杆菌代谢显著的碳源有D-糖二酸、半乳糖醇等9种,氮源有缩二脲、葡萄糖苷酸等11种;解淀粉芽胞杆菌的渗透压和pH环境适应力比烟草青枯病菌强;解淀粉芽胞杆菌具有脱羧酶和脱胺酶的活性。研究表明,2种细菌的代谢表型间存在较大差异,解淀粉芽胞杆菌的碳源、氮源、渗透压及pH代谢表型较烟草青枯病菌的丰富,烟草青枯病菌的磷源、硫源和生物合成途径代谢表型较解淀粉芽胞杆菌的丰富。
To explore the potential of Bacillus amyloliquefaciens X60 as a biocide against tobacco bacterial wilt, different metabolic phenotypes of the two bacteria were compared using the Biolog metabolic phenotyping technique. The results showed that tobacco bacterial wilt and Bacillus amyloliquefaciens could metabolize 19%, 41% carbon source, 43%, 77% nitrogen source, 95%, 86% phosphorus source and 100%, 69% There were 94,91 biosynthetic pathways, 49,95 kinds of osmotic pressure phenotypes and 19,94 pH metabolic phenotypes, respectively. Bacillus amyloliquefaciens significantly metabolized L-pectin, - mannose and other 34 kinds of nitrogen sources of adenosine, cytidine and other 29 species; tobacco bacterial wilt Bacillus than Bacillus amyloliquefaciens significant carbon metabolism are D-saccharic acid, galactitol and other nine kinds of nitrogen sources Biuret and glucuronide; Bacillus amyloliquefaciens's osmotic pressure and pH environment adaptability is stronger than that of tobacco bacterial wilt; Bacillus amyloliquefaciens has decarboxylase and deaminase activities. The results showed that the metabolic phenotypes of the two bacteria were quite different. The carbon source, nitrogen source, osmotic pressure and pH metabolic phenotype of B. amyloliquefaciens were more abundant than those of R. solanacearum, Metabolic phenotype of sulfur source and biosynthetic pathway is more abundant than that of Bacillus amyloliquefaciens.