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目的探讨红细胞达菲(Duffy)血型和艾滋病病毒(HIV)1型感染的关系。方法将彝族HIV高危人群标本分为实验组(HIV感染者)和对照组(未感染HIV者),应用血清学方法进行Duffy血型表型鉴定,使用聚合酶链式反应-序列特异性引物扩增技术(PCR-SSP)进行基因分型,比较两组Duffy血型表型、基因型的分布及其与该地区已知报道的差异,以及不同Duffy血型表型、基因型的HIV感染率。结果共收集彝族HIV高危人群标本427例,其中HIV感染156例(实验组),未感染166例(对照组)。高危人群Duffy血型分布与同地区同民族差异无统计学意义(P>0.05)。感染者与未感染者Duffy血型差异无统计学意义。表型Fy(a~+b~-)、基因型Fya/Fya、Fya/Fyb/Fy占94.41%(304/322),其中实验组146例,对照组158例;表型Fy(a~+b~+)、基因型Fya/Fyb占4.35%(14/322),其中实验组6例,对照组8例;表型Fy(a~-b~+)、基因型Fyb/Fyb占1.24%(4/322),均为实验组;实验组和对照组均未检出Fy(a~-b~-)表型、Fy/Fy、Fyb/Fyb/Fy基因型。两组Duffy血型表型分布比较差异无统计学意义(P>0.05)。Fya~-与Fya~+高危人群,Fyb~+与Fyb~-高危人群HIV-1感染率比较差异均无统计学意义,基因频率为:Fy~a=0.9239(595/644)、Fy~b=0.0342(22/644)、Fy=0.0419(27/644)。结论血清学、分子生物学方法检测结果一致;流行病学调查方面尚不能认为红细胞Duffy血型与HIV-1感染相关。
Objective To investigate the relationship between erythrocyte Duffy blood group and HIV type 1 infection. Methods The HIV high risk population of Yi nationality were divided into experimental group (HIV infected group) and control group (uninfected with HIV group), Duffy blood phenotype was identified by serological method, and PCR amplification was performed by polymerase chain reaction-sequence specific primers (PCR-SSP). The Duffy blood group phenotypes, genotype distributions and their reported differences with the region were compared. The HIV infection rates of different Duffy blood group phenotypes and genotypes were compared. Results A total of 427 HIV-infected HIV patients were collected, of which 156 were HIV-infected (experimental group) and 166 were not infected (control group). The distribution of Duffy blood type in high risk population was not statistically different from that in the same area (P> 0.05). There was no significant difference in Duffy blood type between infected and uninfected people. Fy (a ~ + b ~), genotypes Fya / Fya and Fya / Fyb / Fy accounted for 94.41% (304/322), of which 146 were in the experimental group and 158 in the control group. The genotype Fya / Fyb accounted for 4.35% (14/322), including 6 in the experimental group and 8 in the control group. The phenotype Fy (a ~ -b ~ +) and the genotype Fyb / Fyb accounted for 1.24% (4/322), all of which were experimental groups. No Fy (a ~ -b ~ -), Fy / Fy, Fyb / Fyb / Fy genotypes were detected in the experimental group and the control group. There was no significant difference in the distribution of Duffy blood type between the two groups (P> 0.05). There was no significant difference in the HIV-1 infection rates between Fya ~ - and Fya ~ + high risk population at high risk of Fyb ~ + and Fyb ~, the frequency of Fy ~ a = 0.9239 (595/644), Fy ~ b = 0.0342 (22/644), Fy = 0.0419 (27/644). Conclusions The results of serology and molecular biology are consistent. The results of epidemiological investigation suggest that the erythrocyte Duffy blood type is not associated with HIV-1 infection.